Novel compounds produced by Streptomyces lydicus NRRL 2433 engineered mutants altered in the biosynthesis of streptolydigin

Participation of putative glycoside hydrolases SlgC1 and SlgC2 in the biosynthesis of streptolydigin in Streptomyces lydicus

Two genes of the streptolydigin gene cluster in Streptomyces lydicus cluster encode putative family 16 glycoside hydrolases. Both genes are expressed when streptolydigin is produced. Inactivation of these genes affects streptolydigin production when the microorganism is grown in minimal medium containing either glycerol or d-glucans as carbon source. Streptolydigin yields in S. lydicus were inc...

Biosynthesis of the RNA polymerase inhibitor streptolydigin in Streptomyces lydicus: tailoring modification of 3-methyl-aspartate.

The asparaginyl-tRNA synthetase-like SlgZ and methyltransferase SlgM enzymes are involved in the biosynthesis of the tetramic acid streptolydigin in Streptomyces lydicus. Inactivation of slgZ led to a novel streptolydigin derivative. Overexpression of slgZ, slgM, or both in S. lydicus led to a considerable increase in streptolydigin production.

New macrolide antibiotics produced by mutants from Streptomyces fradiae NRRL 2702.

New pacidamycins produced by Streptomyces coeruleorubidus, NRRL 18370.

were isolated.1^ Here we report the production, isolation, structure elucidation, and biological activities of pacidamycins D (1), 4N (2), and 5T (3). A fresh culture of strain NRRL18730 was inoculated into four 250-ml baffled shake flasks, each containing 50 ml of seed medium consisting of glucose monohydrate 1.0%, soluble starch 1.5%, yeast extract 0.5%, casitone 0.5%, CaCO3 0.1% (pH adjusted...

Potent antifouling compounds produced by marine Streptomyces.

Biofouling causes huge economic loss and a recent global ban on organotin compounds as antifouling agents has increased the need for safe and effective antifouling compounds. Five structurally similar compounds were isolated from the crude extract of a marine Streptomyces strain obtained from deep-sea sediments. Antifouling activities of these five compounds and four other structurally-related ...